To standardize OFTT, you need to answer the following questions:

1. How long after consumption should the sample be taken?
2. What should be the composition of a standardized meal?
3. What level of triglycerides is considered abnormal?

The Copenhagen Heart Study showed the greatest increase in postprandial triglyceride levels at 4 hours after a meal.

Similar results were noted in the Women’s Heart Studies, where measurements 4 hours after a meal were most reliable.

Research on this issue has been going on for 20 years.

A meta-analysis of 113 studies that were performed during the last 20 years was published in 2011 and defines the standardized Oral Fat Tolerance Test.

A group of experts, after a meta-analysis of 113 studies, established the following method of analyzing postprandial triglyceride levels:
1. The most appropriate time to collect a sample is 4 hours after a meal.
2. The Oral Fat Tolerance Test should contain 75 g of saturated fat, 25 g of carbohydrates and 10 g of protein.
3. The test is positive if the triglyceride level is equal to or above 220 mg/dl.

The Oral Fat Tolerance Test involves determining the level of triglycerides within 4 hours after consuming a standardized meal, which is the LIPOTEST meal containing 75 g of saturated fat, 25 g of carbohydrates and 10 g of protein.

*Mihas C, Kolovou GD, Mikhailidis DP, Kovar J, Lairon D, Nordestgaard BG, Ooi TC, Perez-Martinez P, Bilianou H, Anagnostopoulou K, Panotopoulos G. Diagnostic value of postprandial triglyceride testing in healthy subjects: a meta-analysis . Curr Vasc Pharmacol. 2011 May;9(3):271-80